The boos lasted for approximately one minute, CNN reported.
Students simultaneously booed and cheered her introduction.
"We are here today to raise awareness of the opioid epidemic in our country and to educate young lives on how to live a drug-free life", she said.
First Lady Melania Trump received a chilly reception from an audience comprised of mostly middle school and high school students on November 26, as a speech she attempted to give at the University of Maryland was drowned out by boos and protests. He asked the students in the audience to stand if they had lost someone they loved to an overdose.
President Trump has had a hard relationship with Baltimore.
"I know each one of you has hopes and dreams for the future, whether it is college, joining the military, or playing a sport", she continued. "I encourage you to use the resources available in your community so that nothing can stand in your way". Using drugs will only slow you down and prevent you from achieving those goals.
The president provoked a furious backlash in July when he took to Twitter to bash the historic port city of 600,000 people as a "disgusting, rat and rodent infested mess" unfit for humans.
The Mark Wahlberg Youth Foundation partially sponsored the event.
In footage of her speech, boos can be made out from the noise in the background.
The president returned to his criticism in an address to House Republicans at a Baltimore retreat in September.
The president also criticized the late Rep. Elijah Cummings, a Democrat who represented parts of the city in Congress for decades.