"Like so many other women in this case who have already been exposed as liars, this latest completely uncorroborated allegation that is nearly 20 years old will also be shown to be patently false", he said.
Articles published a year ago in The New York Times and The New Yorker detailing his alleged behaviour helped spark the #MeToo movement.
The victim is said to have been a teenager who moved to the U.S. from Poland to pursue an acting career.
When he learned she was interested in acting, the accused allegedly gave her his card and said they'd have lunch to discuss her future.
The woman, who is remaining anonymous as Jane Doe, claims the attacks began when she was 16 years old as an inspiring model.
The woman, identified only as "Jane Doe" in court papers, says she found out at the event that Weinstein was a movie producer.
She alleges that she did not get any work from Weinstein or TWC as she had rebuffed his advances.
Completely distraught, Jane Doe screamed and the producer ultimately let her leave.
In another encounter described in the filing, Weinstein blurted out his sexual attraction to pop star Christina Aguilera, who was being shown on a television, and then allegedly unzipped his trousers and touched his penis.
They continue: "Weinstein threatened and pressured Jane Doe, saying that he had "made" the careers of Penelope Cruz and Gwyneth Paltrow, and that neither would be working without him". Jane Doe fled the room.
The women allege they were assaulted or that they refused his demands for sexual favors, and afterward, promises for roles or picture deals were never kept, says the lawsuit, which also targets heavy hitters in the entertainment industry who the suit alleges abetted Weinstein's behavior.
That case was recently roiled by back-to-back allegations that a New York City police detective acted improperly.
Prosecutors dropped a criminal charge involving a third woman after evidence surfaced that Det.
The amended civil complaint containing Jane Doe's allegations was filed on the last day for lawyers to revise the lawsuit.
A judge had given them until October 31 after expressing scepticism about the strength of their allegations the studio board conspired to cover-up Weinstein's misdeeds by intimidating accusers.
Shockingly, Jane Doe claims Weinstein was at one point distracted by a Christina Aguilera performance on television, said "Wow, I'd really like to fuck that pussy" and proceeded to fondle himself.
After picking her up in his auto for what she thought was a business lunch, she told him she was only 16, the papers say.