The ad includes footage of Luis Bracamontes, a twice-deported immigrant from Mexico sentenced to death in California for killing two police officers.
"After further review, we recognize the insensitive nature of the ad and have chose to cease airing it across our properties as soon as possible", said Joe Benarroch, a spokesperson for the network's advertising sales department, said.
The 30-second ad focuses on the controversial so-called migrant caravan of asylum-seekers making their way to the USA border.
Cut to Monday when, per the New York Times, all three media companies announced that they would no longer be running the ads after the intense backlash.
Mr Trump's campaign manager, Brad Parscale, tweeted that NBC News, CNN and Facebook had chosen "to stand with those ILLEGALLY IN THIS COUNTRY". "President Trump and his allies will protect our border and keep our families safe", according to the ad, which urges viewers to vote Republican.
Fox News quickly followed in NBC's footsteps. "On Monday morning, an NBC Universal spokesperson said the company recognizes 'the insensitive nature of the ad and have made a decision to cease airing it across our properties as soon as possible'".
"The #FakeNewsMedia and #PaloAltoMafia are trying to control what you see and how you think", he said, an apparent reference to Facebook's Silicon Valley headquarters. "While the video is allowed to be posted on Facebook, it can not receive paid distribution".
"So @nbc and @Comcast aired that racist Trump caravan commercial during the football game".