With the visiting bowlers seemingly clueless in their attempts to break the partnership, it took a display of supreme athleticism from Indian skipper Virat Kohli to put an end to their 104-run stand and, yet again, deny Root a century.
Meanwhile the words Kohli appeared to utter in Root´s direction after the run out could yet lead to action by match referee Jeff Crowe, the former New Zealand captain. No fifer for Ashwin but a good bowling performance from Shami.
Only Cook fell in the first session, his front-foot defence defeated by a flawless off-break from Ashwin - repaying his introduction in only the seventh over, despite being India's sole spinner here, with telling drift from round the wicket before the ball turned and dislodged the off-bail.
Click play on the video above to see the best of day two at Edgbaston, as Curran (3-23) removed Murali Vijay and KL Rahul in the space of three balls before dismissing Shikhar Dhawan in his next over.
"He (Kohli) celebrated, and that´s cool", the opening batsman added.
But a brisk innings of 70 in 88 balls, featuring nine fours, was terminated when Bairstow played on to paceman Umesh Yadav as he tried to cut a ball that was too close to him.
But his 11th Test fifty without a hundred since that innings ended in frustrating fashion when, with Yorkshire team-mate Jonny Bairstow calling him for a tight second run, he was run out for 80 by Kohli´s direct hit from midwicket. Serves this outside off, it moves away, Curran flirts with it and gets an edge which Karthik doesn't drop.
Left-arm seamer Sam Curran rocked the Indian top order with triple blow leaving the visitors reeling on 76 for three at lunch on the second day of the first Test.
It was Root's own version of the gesture that signed off the one-day series victory by England at Headingley a fortnight ago. He then had Dawid Malan lbw for eight.