The score was 4-4, 15-0 to Young when he slipped on the baseline and his instant reaction was to hold his face in his hands while lying on the grass.
The talented Australian maverick slipped awkwardly on the grass at the back of the court during a rally at 4-4 in the first set against American Donald Young, succumbing again to a recent, problematic hip injury.
The World No 20 made a decision to keep playing, but he was clearly affected by the injury and, after Young won the first-set tie-break 7-3, Kyrgios trudged to the net to withdraw from the Wimbledon warm-up event.
Kyrgios might be a doubt for Wimbledon, which begins July 3.
"My main goal is to play well at Wimbledon, so I'm going to try and get it better and rehab it and hopefully it settles down", he added.
"I don't really have time to go home or anything. Yeah, I will be playing, for sure".
Asked if he was optimistic of playing at the All England Club in less than a fortnight, Kyrgios said: "Yeah, of course".
The world No.20 clutched initially at his bandaged right knee but he was treated on court after a lengthy delay for left hip soreness.
Nick Kyrgios has consistently performed well during the grass-court season, but a rough fall at Queen's Club on Monday has put his Wimbledon campaign in jeopardy.
"I felt pretty much everything I was feeling a month ago. I had to play my best tennis to get over him", Tsonga said. "I'm sure I'll live", he said.
Asked what he would now do to wile away the time at the house he's renting in Wimbledon with his mother, and girlfriend, Kyrgios quipped: "Dog and Fox", - a reference to a local pub in the affluent suburb.
It has been a hard season for Kyrgios, who has been battling injury and also took a short break from tennis in April following the death of his grandfather.
Grigor Dimitrov, the Bulgarian World No 11, cruised into the second round with a 6-3, 6-1 win against Ryan Harrison of the United States. Shapovalov was fined $7,000 by the ITF.
A medical time-out followed at the next change of ends but the 22-year-old signalled his ongoing discomfort with numerous shakes of his head.