According to the ministry, at the moment the Syrian warplane was hit by the United States fighter, Russia's Aerospace Forces were carrying out missions in Syria's airspace; "However, the coalition command did not use the existing communication line between the air commands of Al Udeid Air Base (Qatar) and Khmeimim Air Base to prevent incidents in Syria's airspace".
The Russian defense ministry also asked the U.S.to provide a full account of why it shot down the Syrian jet.
"Repeated combat actions by US aviation under the cover of counterterrorism against lawful armed forces of a country that is a member of the United Nations are a massive violation of global law and de facto a military aggression against the Syrian Arab Republic", the Russian Defense Ministry said. The shoot-down was the first since American forces began fighting ISIS forces in Syria in 2014.
There are fears Canadian military aircraft operating over Syria could be caught in the middle of a new and potentially explosive dispute between the USA and Russian Federation.
It was the second time Russian Federation suspended a hotline meant to minimize incidents with the U.S.in Syrian airspace.
It is believed to be the first air-to-air kill of a manned aircraft by a U.S. military jet since the Kosovo campaign in 1999.
Every president since George W. Bush has used the AUMF to conduct military operations in countries where it says terror groups are present, including Afghanistan, Iraq, Libya, Somalia, Syria and Yemen.
The new clash comes two months after the USA launched a barrage of Tomahawk missiles at a Syrian airbase that had hosted airplanes responsible for a chemical weapons attack in Idlib province.
The shoot-down comes during a period of heightened tensions between the U.S., Iran, Russia and the Syrian regime.
Sunday's incident reportedly marks the first time the USA has shot down a Syrian regime warplane.
Dunford said he is focused on ensuring the USA can achieve its main objective in Syria - the defeat of Islamic State militants. US -backed forces have encircled the city of Raqqa and captured several districts from ISIS. Use of the hotline continued, however.