The roll call this year, when each state announced its delegate totals from the primary season, affirmed a nomination Clinton locked up weeks ago.
There were tears from some in the audience but the overwhelming sound was enthusiasm.
After a long battle with US Senator Bernie Sanders, Clinton has more than the 2,383 delegates needed to win the vote and be the party's standard-bearer against Republican nominee Donald Trump in the Nov 8 election.
Trump took the usual shots at Clinton during a speech in Charlotte, North Carolina, calling her "Crooked Hillary" and charging that her use of a private email account as secretary of state "put America's entire national security at risk".
Hillary Clinton made history Tuesday night when Democratic delegates formally chose her to become the first woman in the nation's history to be a major party nominee for president.
Within minutes of Sanders speaking, a throng of his supporters walked out of the convention center, waving "Bernie or Bust" signs in the air. Protests outside the convention and intermittent boos of speakers marred the intended message of Democratic unity, although things mostly settled down by the time the day's keynote speakers took the stage.
Vermont's delegation passed up their turn in the vote counting, to secure a place at the end of the line, giving Sanders the opportunity to make the historical motion.
Sen. Barbara Mikulski, U.S. Rep. John Lewis and Na'ilah Amaru, a NY delegate, each offered nominating speeches for Clinton.
During Hillary Clinton's first presidential campaign in 2008, her husband angered some Democrats with dismissive comments about Obama. Liberals, including Sanders supporters, have always been critical of some of his trademark economic policies, such as the North American Free Trade Agreement with Mexico and Canada, and Wall Street deregulation.
Sanders, a 74-year-old USA senator from Vermont, drew a fervent following of youth and liberals during a primary campaign that called for a tough hand on Wall Street and more aggressive steps to counter social inequality.
IL cast 98 votes for Clinton and 74 for Sanders.
Clinton said she'll campaign with Wasserman Schultz in Florida and angered Sanders delegates by appointing her to serve as "honorary chair" of her campaign's 50-state program to help elect Democrats.
She reached the milestone in a roll call vote from all 50 states at the Democratic National Convention (DNC).
Former President Bill Clinton portrayed his wife Hillary on Tuesday as a force for change and a longtime fighter for social justice as he made a case for her historic 2016 bid for the White House.
"What an incredible honor that you have given me".
"Hillary Clinton has the passion and understanding to support grieving mothers", said Sybrina Fulton, whose son Trayvon Martin was killed in 2012.